Cytokinin Induces de Novo Auxin Biosynthesis in Arabidopsis. In the endpoint assays, the electron acceptor DCPIP (Sigma‐Aldrich, Raleigh, NC, USA) was applied in McIlvaine buffer (100 m m citric acid and 200 m m Na 2 HPO 4) at pH 7.5. effect, while cytokinin ribosides such as trans-zeatin riboside, isopentenyladenosine, and benzylaminopurine riboside impair the viability of normal and neoplastic cells, apart ... reagents and all solvents were purchased from Sigma-Aldrich (St. 3401 Correspondence to: Pierangela Ciuffreda, Dipartimento di Scienze Auxin and cytokinin control formation of the quiescent centre in the adventitious root apex of arabidopsis F. Della Rovere †, L. Fattorini , S. D’Angeli, A. Veloccia, G. Falasca and M. M. Altamura* Department of Environmental Biology, Sapienza University of Rome, Italy They claimed that “It is difficult to unify the cytokinin ... Sigma-Aldrich both listed BA and BAP, the product could only be purchased as BAP. To examine the effect of shoot-derived auxin on the regeneration process, 5 µM auxin polar transport inhibitor 2,3,5-triiodobenzoic acid (TIBA) (Fluka, Chemie GmbH, Buchs, Switzerland) was applied in a low gelling agarose (Sigma-Aldrich) droplet at the junction of the shoot to the root. The chemicals kinetin and sodium arsenite were obtained from Sigma-Aldrich (Dorset, Germany). We showed that DOF2.1 acts downstream of the TMO5/LHW-dependent cytokinin response and, together with its closest homologs, controls vascular proliferation. Photosynthetic activity is affected by exogenous and endogenous inputs, ... (Sigma-Aldrich). In the case of Nacalai Tesque, both BA and BAP were listed, but BA was confusingly 4-Nitroquinoline 1-oxide (NQO-sc256815) was purchased from Santa Cruz Biotechnology (Heidelberg, Germany), dihydroethidium (DHE) was purchased from Merck Biosciences GmbH (Schwalbach, Germany). ... (Sigma-Aldrich) was prepared as 10 mM, AVG (Fluka) as 10 mM, 2,4-D (Duchefa) ... Cytokinin regulates root meristem activity via modulation of the polar auxin transport. Abscisic acid (ABA) and cytokinin antagonistically regulate many developmental processes and environmental stress responses in plants. Moreover, the promotion of rice seminal root growth was correlated with a decrease ... Zeatin (Z) (Sigma-Aldrich Trading Co., Ltd., Shanghai, China), a mixture of trans-Z (tZ) and cis-Z (cZ), was dissolved in dimethyl sulfoxide (DMSO) and diluted to the desired concentration with Effects of nutrient media, different cytokinin types and their concentrations on in vitro multiplication of G × N15 (hybrid of almond × peach) vegetative rootstock. However, the molecular mechanism underlying this antagonism remains poorly defined. The 600‐μL reaction mixture contained 0.5 m m DCPIP, 0.25 m m cytokinin and 1.6 μ m LuCKX1.1. of cytokinin (CK). Multiple hormones including cytokinin and auxin have been implicated in the regulation of root development. cytokinin ribosides including a series of adenosine analogs differently substituted in the N6 position, along with some ... reagents and all solvents were purchased from Sigma-Aldrich (St. 3401 Correspondence to: Pierangela Ciuffreda, Dipartimento di Scienze cytokinin nomenclature and corresponding abbreviations.
SPINDLY (SPY) is a negative regulator of gibberellin (GA) responses; however, spy mutants exhibit various phenotypic alterations not found in GA-treated plants.
... (MS) medium containing 0.5 mg l −1 of BAP (Sigma–Aldrich, Steinheim, Germany). Timely removal of˜exogenous cytokinin and˜the˜prevention of˜auxin ... (Sigma-Aldrich,St.Louis,Missouri,UnitedStates), 2.68µM6-benzyladenine(BA)(Sigma-Aldrich),1.48µM isopentenyl-adenine(IPA)(Sigma-Aldrich)for3days(ifnot indicatedotherwise)thenonARMIIrmedium(Mártonand Ovule culture was performed according to the method described previously by Beasley and Ting (1973).Indole-3-acetic acid sodium salt (IAA) and gibberellic acid potassium salt (GA 3; both Sigma-Aldrich) were dissolved in distilled water and filter-sterilized to obtain stock solutions of 50 mM and 10 mM, respectively. Ovule culture.